Nutrient Mixture F-12 Ham, with L-glutamine is widely used in cell culture applications to support the growth and maintenance of various mammalian cells. It is particularly effective for culturing primary cells and cell lines, including Chinese hamster ovary (CHO) cells, which are commonly used in biopharmaceutical production. The mixture provides essential nutrients, vitamins, amino acids, and L-glutamine, which serves as a critical energy source for cells. It is often utilized in research laboratories for studying cell behavior, drug screening, and toxicity testing. Additionally, it is employed in tissue engineering and regenerative medicine to create optimal conditions for cell proliferation and differentiation. Its balanced formulation makes it suitable for both adherent and suspension cell cultures, ensuring consistent and reliable results in experimental and industrial settings.