Widely used in cell culture applications, this medium supports the growth and maintenance of a variety of mammalian cells, including primary cells, cell lines, and stem cells. Its high glucose concentration provides an energy source for cells with high metabolic demands, while L-glutamine serves as an essential nutrient for protein synthesis and cell proliferation. The inclusion of HEPES buffer ensures pH stability, particularly in open or non-CO2 environments, making it suitable for experiments requiring extended incubation periods. The absence of sodium pyruvate and phenol red makes it ideal for sensitive assays, such as fluorescence microscopy or enzyme activity studies, where these components could interfere with results. Commonly employed in research areas like cancer biology, immunology, and drug discovery, it is also utilized in the production of biologics and vaccines.