Dulbecco’s Modified Eagle’s Medium (DMEM) - low glucose, with L-glutamine and sodium pyruvate, is widely used in cell culture applications. It is particularly suitable for growing a variety of mammalian cells, including primary fibroblasts, neurons, and certain cancer cell lines. The low glucose formulation helps reduce metabolic stress in cells, making it ideal for studies requiring controlled glucose levels. The inclusion of L-glutamine supports protein synthesis and cell proliferation, while sodium pyruvate provides an additional energy source, enhancing cell viability and growth. This medium is commonly employed in research areas such as cell biology, drug screening, and toxicology studies, where maintaining consistent and optimal cell health is critical. Its versatility and balanced nutrient composition make it a staple in laboratories for routine cell culture and experimental work.