L-15 Medium (Leibovitz), with L-glutamine, is widely used in cell culture applications, particularly for maintaining and growing cells that require a CO2-independent environment. It is suitable for primary cell cultures and cell lines, especially those derived from mammalian tissues. This medium is ideal for research involving virus production, vaccine development, and studies requiring cell metabolism analysis. Its formulation supports the growth of cells in ambient air, making it convenient for labs without CO2 incubators. Additionally, it is often employed in experiments involving live-cell imaging, as it maintains stable pH levels without the need for a CO2 buffer system. The inclusion of L-glutamine enhances cell growth and viability, making it a reliable choice for various biomedical and biotechnological applications.