RPMI-1640 Medium, with L-glutamine and without phenol red, is widely used in cell culture applications, particularly for growing and maintaining mammalian cells. Its formulation supports the growth of a variety of cell types, including hybridomas, lymphocytes, and other suspension cells. The absence of phenol red makes it suitable for experiments where colorimetric interference must be avoided, such as fluorescence microscopy or assays involving optical detection methods. This medium is often utilized in immunological studies, cancer research, and drug screening due to its ability to maintain cell viability and proliferation. Additionally, it is commonly employed in the production of monoclonal antibodies and in studies requiring precise monitoring of cellular responses without the influence of phenol red.