McCoy's 5A Medium, with L-glutamine, is widely used in cell culture applications, particularly for growing primary cells and established cell lines. It is especially suitable for culturing fibroblasts, bone marrow, and other cells derived from soft tissues. The medium supports the growth of a variety of cell types, including those used in cancer research, due to its balanced nutrient composition and ability to maintain cell viability. It is commonly employed in studies involving cell proliferation, differentiation, and drug screening. Additionally, its formulation makes it ideal for use in hybridoma production and other biotechnological applications requiring stable cell growth conditions.