RPMI 1640 Medium is widely used in cell culture applications, particularly for growing human and mammalian cell lines. It is especially effective for culturing lymphocytes, hybridomas, and leukemia cells, making it a staple in immunology and cancer research. The medium supports cell proliferation and maintains cellular function in suspension and adherent cultures. It is commonly used in studies involving drug screening, vaccine development, and monoclonal antibody production. Due to its balanced formulation, it is also suitable for use in cytotoxicity assays, cell fusion experiments, and metabolic studies. This specific formulation is L-glutamine-free and sodium pyruvate-free, contains HEPES for buffering, phenol red as a pH indicator, glucose, and sodium bicarbonate. RPMI 1640 is often supplemented with fetal bovine serum, L-glutamine, and antibiotics to enhance cell growth and prevent contamination. Its versatility makes it a preferred choice in both research laboratories and biopharmaceutical manufacturing.