Used in cell culture applications requiring a calcium-free environment, this medium supports the growth of sensitive cell types such as epithelial and endothelial cells, especially in studies involving calcium signaling or mineralization processes. It is commonly used in hybridoma formation, neural cell cultures, and stem cell differentiation where precise control of extracellular calcium is critical. The absence of calcium allows researchers to supplement the medium with controlled amounts of calcium or chelating agents, making it ideal for experimental manipulation of calcium-dependent pathways. It is also used in transfection protocols and in vitro toxicology studies where calcium interference must be avoided.