Widely used in cell culture applications to support the growth of a variety of mammalian cells, including primary cells, stem cells, and established cell lines. Ideal for research involving cell proliferation, differentiation, and protein production due to its high glucose content, which provides ample energy for metabolically active cells. Commonly employed in virology studies, vaccine development, and biopharmaceutical production. Suitable for experiments requiring precise control over media components, as it lacks HEPES, double antibodies, potassium ions, and serum, allowing for customization based on specific research needs. Often utilized in 3D cell culture systems, tissue engineering, and drug screening assays.