Used primarily in cell culture applications, this medium supports the growth and maintenance of a variety of mammalian cells. It is particularly useful in research involving immunology, cancer studies, and virology, where the absence of calcium nitrate, L-glutamine, and phenol red is required. The exclusion of these components allows for greater control over experimental conditions, making it suitable for studies involving calcium-sensitive processes, customized nutrient supplementation, or fluorescence-based assays where phenol red could interfere. It is also employed in hybridoma technology and the production of monoclonal antibodies. Additionally, its formulation is ideal for experiments requiring a defined and consistent environment, such as drug screening and toxicity testing.