DMEM/F12 is widely used in cell culture applications, particularly for growing a variety of mammalian cells. Its balanced formulation supports the growth of both primary and established cell lines, making it a versatile medium in research and biopharmaceutical production. It is commonly employed in studies involving cell proliferation, differentiation, and toxicity testing. The medium is also utilized in tissue engineering and regenerative medicine, where it helps maintain the viability and functionality of cells. Additionally, DMEM/F12 is often used in the production of biologics, such as monoclonal antibodies and recombinant proteins, due to its ability to sustain high cell densities and productivity. Its compatibility with serum-free and chemically defined conditions makes it suitable for applications requiring precise control over cell culture environments.