Z-Asp-CH2-DCB is primarily used in biochemical research as a potent and selective inhibitor of caspases, a family of cysteine proteases involved in programmed cell death (apoptosis). Its application is crucial in studying the mechanisms of apoptosis and related pathways in cellular biology. Researchers utilize this compound to inhibit caspase activity, allowing them to dissect the roles of specific caspases in various cellular processes, including inflammation, cell differentiation, and disease progression. Additionally, Z-Asp-CH2-DCB is employed in drug discovery and development to screen and evaluate potential therapeutic agents targeting caspase-mediated diseases, such as neurodegenerative disorders, cancer, and autoimmune conditions. Its specificity and efficacy make it a valuable tool for understanding and manipulating apoptotic pathways in both in vitro and in vivo studies.