Nyssoside is primarily used in research settings for its bioactive properties, particularly in studies related to cardiovascular function and cellular signaling. It exhibits cardiotonic effects by influencing ion transport in heart muscle cells, making it a compound of interest in the development of treatments for heart failure and related conditions. Researchers also investigate its potential as a modulator of sodium-potassium ATPase activity, which plays a critical role in maintaining cellular electrochemical gradients. Due to its structural similarity to certain cardiac glycosides, nyssoside is studied for its ability to enhance myocardial contractility, though its use remains limited to experimental models and pharmacological research.