Monodisperse carboxy silica microspheres are widely used in biomedical research and diagnostics due to their uniform size and functional carboxyl groups. They serve as excellent carriers for drug delivery systems, enabling targeted and controlled release of therapeutic agents. In diagnostics, these microspheres are employed in immunoassays and biosensors, where their surface can be modified with antibodies or other biomolecules for precise detection of target analytes. Additionally, they are utilized in chromatography as stationary phases, offering high resolution and efficiency in separating complex mixtures. Their biocompatibility and ease of surface modification make them valuable in tissue engineering and regenerative medicine for scaffolding and cell culture applications.