Monodisperse carboxy silica microspheres are widely used in biomedical research for drug delivery systems due to their uniform size and surface functionalization. Their carboxyl groups enable easy conjugation with biomolecules, making them ideal for targeted therapy and diagnostic applications. In chromatography, these microspheres serve as stationary phases, providing high-resolution separation of complex mixtures. They are also employed in immunoassays as solid supports for antibody immobilization, enhancing detection sensitivity and specificity. Additionally, their use in calibration standards for particle size analyzers ensures accurate measurements in various industries. The microspheres are also utilized in material science for creating advanced composites with tailored properties.