Monodisperse carboxy silica microspheres are widely used in biomedical research for their uniform size and functional carboxyl groups. They serve as excellent carriers for drug delivery systems, enabling precise targeting and controlled release of therapeutic agents. In diagnostics, these microspheres are employed as solid supports for immobilizing proteins, antibodies, or nucleic acids, enhancing the sensitivity and accuracy of assays like ELISA and flow cytometry. Their biocompatibility and surface reactivity make them ideal for cell separation and sorting applications, particularly in isolating specific cell populations. Additionally, they are utilized in chromatography as stationary phases for efficient separation of biomolecules. In material science, these microspheres act as building blocks for creating ordered structures or coatings with tailored properties.