Used as a stable isotope-labeled tracer in metabolic studies, particularly in research involving choline metabolism and its role in liver function, cell membrane synthesis, and neurotransmitter production. The 13C label allows for precise tracking using mass spectrometry or NMR, enabling accurate measurement of metabolic fluxes in biological systems. Commonly applied in clinical and pharmaceutical research to study diseases such as fatty liver, neurological disorders, and cancer. Also utilized in isotopic labeling experiments to assess nutrient absorption and bioavailability in humans and animals.