Used as a stable isotopically labeled compound in metabolic research, Acetaldehyde-¹³C₂ enables precise tracing of metabolic pathways, particularly in studies involving alcohol metabolism and the citric acid cycle. It serves as a key tracer in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) to analyze biochemical transformations and enzyme mechanisms. Its labeled carbon atoms allow researchers to track the fate of acetaldehyde in biological systems, aiding in the investigation of oxidative stress, detoxification processes, and carcinogenesis related to alcohol consumption. It is also employed in the synthesis of labeled compounds for pharmaceutical and environmental testing.