Blue Dextran is widely used in biochemical and molecular biology research as a marker for gel filtration chromatography. Its high molecular weight makes it ideal for determining the void volume of a column, which helps in calibrating and understanding the separation characteristics of the column. Additionally, it is employed in studies of cell permeability and membrane transport, where its size and color make it easy to track and analyze. In protein purification processes, Blue Dextran serves as a visual aid to monitor the flow and efficiency of separation techniques. It is also used in educational laboratories to demonstrate principles of chromatography and molecular sieving. Furthermore, Blue Dextran is utilized in physiological studies to assess kidney function, serving as a marker for glomerular filtration rate (GFR) in animal models by measuring its excretion rate.