Blue Dextran is widely used in biochemical and molecular biology research as a marker or tracer. It is particularly valuable in gel filtration chromatography for determining the void volume of columns, as its large molecular size helps in calibrating and assessing the performance of the column. Additionally, it is employed in studies related to membrane permeability and vascular leakage, where its blue color allows for easy visualization and tracking. In protein purification processes, Blue Dextran aids in monitoring the separation and elution of molecules based on size. Its applications also extend to medical research, where it is used to study blood-brain barrier function and other physiological processes involving macromolecular transport.