Polysucrose 400 is widely used in biological and medical research as a density gradient medium. It is particularly valuable in cell separation techniques, such as isolating specific cell types from blood or tissue samples. Its high molecular weight and solubility make it ideal for creating gradients that allow cells to separate based on density during centrifugation. Additionally, it is used in the purification of viruses, organelles, and other subcellular components due to its non-toxic and inert properties. Polysucrose 400 is also employed in diagnostic assays and as a stabilizer in various biochemical applications. Its ability to maintain osmotic balance without interfering with cellular functions makes it a preferred choice in laboratory settings.