Polysucrose 100 is widely used in biochemical and medical research as a density gradient medium for cell separation and purification. It is particularly effective in isolating specific cell types, such as lymphocytes, from complex mixtures like blood or tissue samples. Its high molecular weight and inert nature make it ideal for creating stable gradients without interfering with cellular integrity. Additionally, it is employed in organelle isolation, enabling researchers to study mitochondria, nuclei, and other subcellular components. Its non-toxic and biocompatible properties also make it suitable for use in vaccine development and diagnostic applications, where it aids in the separation of antigens or antibodies.