N,N',N''-Triacetylchitotriose is widely used in biochemical research as a substrate to study the activity of chitinases, enzymes that break down chitin. It helps in understanding the enzymatic mechanisms and kinetics involved in chitin degradation, which is crucial for developing antifungal agents and biopesticides. Additionally, it serves as a model compound in glycobiology to investigate carbohydrate-protein interactions, particularly in the context of immune responses and pathogen recognition. Its role in probing the structure and function of chitin-binding proteins also makes it valuable in studies related to plant defense mechanisms and microbial pathogenesis.