Used as a substrate in enzymatic assays to detect β-glucuronidase activity, which is a marker for certain bacterial infections, including E. coli. It is also employed in histochemistry and microbiology for identifying and studying enzyme activity in cells and tissues. In research, it helps in the development of diagnostic tools and assays for monitoring bacterial contamination in food and water. Additionally, it serves as a reagent in molecular biology for studying gene expression and enzyme kinetics. Its application extends to drug development, where it aids in screening compounds that modulate β-glucuronidase activity.