IPTG solution is widely used in molecular biology and biotechnology for the induction of protein expression in recombinant bacterial systems. It is particularly effective in inducing the lac operon in E. coli, allowing for the production of target proteins under the control of the lac promoter. Researchers utilize IPTG to trigger the expression of genes cloned into plasmids containing the lac operator, enabling the study and purification of proteins of interest. It is also employed in high-throughput screening and industrial protein production due to its reliability and efficiency in inducing gene expression. Additionally, IPTG is used in experiments involving β-galactosidase assays as a non-metabolizable analog of lactose, ensuring consistent and controlled induction without being consumed by the cells.