Dextran blue 2000 is widely used in biochemical and medical research as a marker or tracer. Its high molecular weight and blue color make it ideal for studying filtration processes, particularly in kidney function tests. It is also employed in gel filtration chromatography to determine the void volume of columns, aiding in the separation and analysis of macromolecules. Additionally, it serves as a visual aid in electrophoresis and other laboratory techniques to track migration patterns. In medical applications, it is used to assess blood-brain barrier permeability and to study vascular leakage in experimental models. Its inert nature and stability make it a reliable tool in various diagnostic and research settings.