D-Xylulose is primarily used in biochemical research to study carbohydrate metabolism, particularly in pathways like the pentose phosphate pathway. It serves as a key intermediate in understanding how sugars are processed and converted into energy or other metabolic products in living organisms. Additionally, it is utilized in the development of diagnostic tools for metabolic disorders, such as rare genetic conditions affecting sugar metabolism. In the food industry, D-Xylulose is explored for its potential as a low-calorie sweetener or as a component in functional foods aimed at improving metabolic health. Its role in enzymatic studies also aids in the development of industrial bioprocesses for producing biofuels or other bio-based chemicals.