D-Ribono-1,4-lactone is widely utilized in the pharmaceutical industry as a key intermediate in the synthesis of various nucleoside analogs, which are crucial for antiviral and anticancer drugs. Its role in the production of ribavirin, an antiviral medication, highlights its importance in treating viral infections such as hepatitis C and respiratory syncytial virus. Additionally, it serves as a precursor in the synthesis of riboflavin (vitamin B2), essential for metabolic processes in the body. In research, it is employed in the study of carbohydrate chemistry and enzymatic reactions, aiding in the development of new biochemical pathways and therapeutic agents. Its application extends to the food industry as a nutritional supplement, contributing to the fortification of products with essential vitamins.