D-Mannosamine hydrochloride is primarily utilized in biochemical research for the study of glycosylation processes. It serves as a precursor in the synthesis of sialic acids, which are crucial components of glycoproteins and glycolipids. These compounds play significant roles in cell-cell interactions, immune response modulation, and pathogen recognition. Additionally, it is employed in the development of glycoconjugate vaccines and therapeutic agents targeting diseases influenced by glycosylation abnormalities, such as cancer and infectious diseases. Its application extends to the production of bioactive molecules used in studying metabolic pathways and enzyme functions related to carbohydrate metabolism.