Used in biochemical research to study enzyme kinetics, particularly those involved in glycolysis and photosynthesis. It serves as a key intermediate in metabolic pathways, helping to understand energy production and conversion in cells. Applied in the development of diagnostic assays to measure enzyme activity. Also utilized in the production of synthetic peptides and as a substrate in enzymatic reactions to investigate metabolic disorders. Its role in metabolic studies aids in advancing knowledge of cellular respiration and plant biochemistry.