Neoagarotetraose is used in cosmetic formulations for its moisturizing, skin-conditioning, and anti-inflammatory properties. It helps improve skin hydration and elasticity by forming a protective film that reduces water loss, making it suitable for anti-aging creams, serums, and products for sensitive or acne-prone skin due to its gentle, non-irritating nature. It is also employed in dietary supplements to promote gut health as a prebiotic, supporting the growth of beneficial gut microbiota, balancing the microbiome, and enhancing digestive function. Additionally, it is investigated for applications in wound healing and tissue engineering owing to its biocompatibility and ability to support cell growth. In research settings, it serves as a biomarker or substrate in enzymatic studies involving agarose metabolism.