N-Acetyl-D-galactosamine is widely used in biomedical research and therapeutic development due to its role as a key component in glycoproteins and glycolipids. It serves as a building block in the synthesis of complex carbohydrates involved in cell signaling and recognition processes. This compound is particularly important in the study of glycosylation patterns associated with cancer, where abnormal surface glycans containing N-Acetyl-D-galactosamine are biomarkers for certain tumor types. It is also employed in the development of glycoconjugate vaccines, helping to elicit immune responses by mimicking natural carbohydrate antigens. In regenerative medicine and cell culture, it supports the growth and differentiation of stem cells, especially in protocols involving induced pluripotent stem cells. Additionally, it acts as a precursor for synthesizing bioactive molecules and is used in assays to investigate enzyme activity, such as glycosyltransferases and glycosidases involved in metabolic disorders.