Used in biochemical research as a chromogenic substrate for detecting β-glucosidase enzyme activity. When the enzyme cleaves the glycosidic bond, it releases 4-aminophenol, which can be easily detected through color development in the presence of oxidizing agents. This makes it valuable in enzyme assays, diagnostic procedures, and studies involving glycosidase expression in biological samples. Also employed in the development of biosensors and in monitoring enzymatic kinetics in pharmaceutical and clinical laboratories.