N-Acetyl-D-lactosamine is widely used in biochemical research, particularly in the study of glycobiology. It serves as a key intermediate in the synthesis of complex carbohydrates and glycoconjugates, which are essential for understanding cell signaling and communication. This compound is also utilized in the development of glycoprotein-based therapeutics, as it plays a role in modulating immune responses and improving drug efficacy. Additionally, it is employed in the production of oligosaccharides for use in nutritional supplements and infant formulas, mimicking the structure of human milk oligosaccharides to support gut health and immunity.