1-Kestoheptaose is primarily utilized in the field of biochemistry and nutrition research due to its unique structure as a seven-carbon sugar. It serves as a valuable substrate for studying enzymatic activities, particularly those of glycosidases and glycosyltransferases, which are crucial in carbohydrate metabolism. This oligosaccharide is also used in the development of prebiotic formulations, as it can selectively stimulate the growth of beneficial gut microbiota, promoting digestive health. Additionally, 1-kestoheptaose finds applications in the synthesis of complex carbohydrates and glycoconjugates, aiding in the exploration of their biological functions and potential therapeutic uses. Its role in modulating immune responses and enhancing gut barrier function is also under investigation, making it a promising candidate for functional food and pharmaceutical industries.