Manninotriose is primarily utilized in the field of biochemistry and plant physiology research. It serves as a key compound in studying carbohydrate metabolism and the enzymatic processes involved in the breakdown and synthesis of complex sugars. Researchers use it to understand the role of oligosaccharides in plant stress responses, particularly in relation to drought and salinity tolerance. Additionally, manninotriose is employed in the development of functional foods and nutraceuticals, where it acts as a prebiotic to promote the growth of beneficial gut microbiota. Its application extends to the production of specialty chemicals and bio-based materials, leveraging its properties as a renewable and sustainable resource.