Used as a key intermediate in biochemical assays and metabolic studies, particularly in the investigation of glycolysis and the pentose phosphate pathway. Serves as a substrate for enzymes such as glucose-6-phosphate dehydrogenase in diagnostic test kits for measuring enzyme activity in clinical and research laboratories. Employed in cell culture media to support energy metabolism in certain specialized systems. Also utilized in enzymatic synthesis where phosphorylated sugar donors are required. Its stability and solubility make it suitable for in vitro studies of glucose metabolism and insulin signaling pathways.