Used primarily as a photolabile protecting group reagent in organic synthesis, especially in the preparation of peptides and nucleotides. The nitrobenzyl-based group allows for controlled release of protected functional groups upon exposure to UV light, enabling precise spatial and temporal activation in chemical reactions. Commonly applied in solid-phase synthesis and biochemical assays where light-triggered deprotection is required. Also utilized in the development of caged compounds for studying biological processes in live cells.