Used as a hypoxia marker in biological research, particularly in cancer studies. It binds selectively to cells with low oxygen levels, allowing researchers to identify and quantify hypoxic regions in tumors. This helps in evaluating tumor aggressiveness and response to therapy, as hypoxia is linked to resistance in radiation and chemotherapy. Commonly detected using immunohistochemistry in tissue samples, it supports the development and assessment of hypoxia-targeting drugs and treatment strategies.