Pyridostatin trifluoroacetate is primarily used in biochemical and biomedical research as a potent and selective stabilizer of G-quadruplex DNA structures. These non-canonical DNA formations are found in regions of the genome such as telomeres and oncogene promoters. By stabilizing G-quadruplexes, Pyridostatin modulates gene expression and inhibits telomerase activity, making it a valuable tool for studying cancer cell proliferation and senescence. It has been employed in studies targeting cancers where oncogenes like c-MYC, KRAS, and BCL-2 are overexpressed, as stabilization of G-quadruplexes in their promoter regions can downregulate their transcription. Additionally, Pyridostatin trifluoroacetate is used to investigate DNA damage responses and genomic instability linked to G-quadruplex formation. Due to its cell permeability and selectivity, it serves as a lead compound in the development of novel anticancer therapeutics. It is also utilized in epigenetic and nucleic acid structural studies to probe the functional roles of secondary DNA structures in regulation and disease.