NSC49652 is primarily used in scientific research as a small molecule inhibitor, particularly in studies related to cell cycle regulation and cancer biology. It has been investigated for its ability to interfere with specific protein-protein interactions that are critical in tumor growth and progression. Due to its inhibitory activity, it serves as a tool compound in probing signaling pathways, especially those involving transcriptional regulation and DNA damage response. Its application is largely confined to in vitro and preclinical research settings, where it helps validate potential therapeutic targets and assess mechanistic pathways in oncology and molecular pharmacology.