Used primarily in biomedical research to inhibit Rho GTPases, particularly Rac1, which plays a key role in cell motility, proliferation, and signaling. It is widely applied in studies related to cancer, where blocking Rac1 activity can suppress tumor cell invasion and metastasis. Also utilized in cardiovascular research to explore roles of Rac1 in endothelial function and vascular remodeling. Commonly employed in cell-based assays to probe cytoskeletal dynamics, including lamellipodia formation and cell migration. Its selectivity makes it a valuable tool for dissecting signaling pathways involving Rac without affecting closely related GTPases like Cdc42 or RhoA.