Myoseverin is primarily used in biological research to study cell division and muscle cell regeneration. It has the unique ability to induce multinucleated muscle cells to re-enter the cell cycle, effectively reversing their differentiated state. This makes it a valuable tool for investigating cellular dedifferentiation and tissue repair mechanisms. Researchers also explore its potential in regenerative medicine, particularly in conditions involving muscle degeneration. Additionally, myoseverin is used to probe the molecular pathways involved in mitosis and cytoskeletal reorganization, offering insights into cancer biology and developmental processes.