GSK621 is primarily used as a potent and selective AMP-activated protein kinase (AMPK) activator in biomedical research. It is widely applied in studies related to cellular energy homeostasis, metabolic regulation, and cancer cell metabolism. Researchers use GSK621 to induce AMPK-dependent signaling pathways, which helps in understanding the role of AMPK in inhibiting cell proliferation, particularly in various cancer models such as lung, breast, and liver cancer. Its ability to trigger autophagy and suppress mTOR signaling makes it a valuable tool in investigating cell survival and death mechanisms. Additionally, GSK621 has been explored in metabolic disease research for its potential to improve insulin sensitivity and glucose uptake in cellular models, highlighting its relevance in diabetes-related studies. Due to its cell permeability and specificity, it is commonly employed in in vitro assays to modulate AMPK activity and assess downstream effects on metabolism and growth.