Used in pharmaceutical research as a fluorescent probe due to the benzo[c][1,2,5]oxadiazole (benzofurazan) moiety, which exhibits strong fluorescence. This property allows real-time tracking of cellular uptake and distribution in biological systems. The brominated biphenyl group enhances binding affinity to specific protein targets, making it useful in the development of enzyme inhibitors. The D-serinate ester component improves cell membrane permeability, facilitating intracellular delivery. Commonly applied in studies involving protease activity and receptor-ligand interactions, especially in cancer and neurodegenerative disease models. Also employed in high-throughput screening assays where detectability and stability are critical.