AU1235 is primarily used as a selective inhibitor in biochemical research, particularly in studies involving kinase signaling pathways. It demonstrates potent activity against specific tyrosine kinases, making it a valuable tool for investigating cellular proliferation, apoptosis, and immune response mechanisms. Due to its high selectivity and cellular permeability, AU1235 is often employed in in vitro assays to modulate kinase activity and assess downstream effects in cancer cell lines and inflammatory models. It has also been utilized in early-stage drug discovery to validate therapeutic targets, especially in oncology and autoimmune disease research. Its application supports the development of more effective and targeted pharmacological agents.