A-674563 is a selective inhibitor of Akt kinase, particularly targeting the Akt1 and Akt2 isoforms, making it a valuable tool in biochemical and pharmacological research focused on signal transduction pathways. It is primarily used in studies related to the PI3K/Akt/mTOR pathway, which plays a critical role in cell survival, proliferation, and metabolism. Due to its inhibitory action, A-674563 is employed to investigate cancer cell growth and apoptosis, especially in models of tumors with hyperactive Akt signaling. Researchers use it to assess the downstream effects of Akt inhibition, such as reduced phosphorylation of target proteins and suppression of tumor progression in vitro and in vivo. Its selectivity allows for more precise modulation of Akt activity compared to broader kinase inhibitors, supporting its use in mechanistic studies and early-stage drug development for oncology and metabolic diseases.