AG-1024 is primarily used in research settings as a selective inhibitor of the insulin-like growth factor 1 receptor (IGF-1R) and insulin receptor (IR) tyrosine kinases. It is employed to study signaling pathways involved in cell growth, proliferation, and survival, particularly in cancer biology. Researchers use AG-1024 to investigate the role of IGF-1R in tumor development, as its inhibition can lead to reduced cancer cell viability and increased apoptosis in various cell lines. It has been applied in studies on breast, prostate, and lung cancers, among others, to assess the therapeutic potential of blocking IGF-1R-mediated signaling. Additionally, AG-1024 serves as a tool compound for exploring metabolic pathways and insulin resistance mechanisms, though its use remains confined to preclinical research due to lack of clinical development.