Hesperadin is primarily used in research settings as a potent and selective inhibitor of Aurora B kinase, a key enzyme involved in cell division. Its application is significant in studying the mechanisms of mitosis and the role of Aurora B in chromosomal alignment and cytokinesis. Researchers utilize Hesperadin to investigate its effects on cancer cells, as Aurora B is often overexpressed in various malignancies. By inhibiting this kinase, Hesperadin helps in understanding the potential for targeting Aurora B in cancer therapy, particularly in halting the proliferation of tumor cells. Additionally, it serves as a tool in exploring cell cycle regulation and the development of novel anti-cancer drugs.