M2I-1 is primarily utilized in research settings as a potent and selective inhibitor of MDM2 (Mouse Double Minute 2 homolog). Its application is significant in the study of cancer biology, particularly in understanding the p53 tumor suppressor pathway. By inhibiting MDM2, M2I-1 helps to stabilize and activate p53, which plays a crucial role in regulating cell cycle arrest, apoptosis, and DNA repair mechanisms. This makes M2I-1 a valuable tool in preclinical studies aimed at developing novel cancer therapies, especially for cancers where p53 function is compromised. Additionally, it is used to explore the therapeutic potential of targeting the MDM2-p53 interaction in various cancer models.